Neuroprotective and Antioxidant Properties of Cholestero Nitrone ChN2 and QuinolylNitrone QN23 in an Experimental Model of Cerebral Ischemia: Involvement of Necrotic and Apoptotic Cell Death

Ischemic stroke is the leading cause of disability and second death worldwide. However, current therapeutic strategies are scarce limited efficacy. The abundance information available on molecular pathophysiology ischemic has sparked considerable interest in developing new neuroprotective agents that can target different events cascade may be used combination with existing treatments. In this regard, nitrones represent a very promising alternative due to their renowned antioxidant anti-inflammatory effects. study, we aimed further investigate effects two nitrones, cholesteronitrone 2 (ChN2) quinolylnitrone 23 (QN23), which have previously shown great potential for treatment stroke. Using an experimental vitro model cerebral ischemia, compared anti-necrotic, anti-apoptotic, properties those three reference compounds. Both ChN2 QN23 demonstrated significant (EC50 = 0.66 ± 0.23 μM EC50 2.13 0.47 μM, respectively) comparable homo-bis-nitrone 6 (HBN6) N-acetylcysteine (NAC) superior α-phenyl-N-tert-butylnitrone (PBN). While primarily derived from nitrones’ anti-necrotic capacities, anti-apoptotic at high concentrations powers—especially case QN23—also contribute